The inclusion level of a phytogenic premix (PP) (Anco FIT Poultry) was studied for its effects on broiler growth performance, nutrient digestibility and total antioxidant capacity (TAC) of meat and liver. In addition, gene expression of antioxidant enzymes CAT, SOD and GPx was profiled along the broiler intestine.
A total of 500 one-day-old Cobb broiler chickens were assigned into 4 treatments, with 5 replicates of 25 chickens each. Basal diets were formulated to meet starter (1 to 10d), grower (11 to 22d) and finisher (23 to 42d) growth phase requirements. Depending on PP inclusion level (0, 750, 1000 and 2000 mg/kg diet), treatments were: PP-0, P-750, PP-1000 and PP-2000. Feed and water were available ad libitum.
Data were analyzed by ANOVA, taking the treatment as fixed effect. Statistical significant effects (P≤0.05) were further analyzed and means were compared using Tukey HSD test. In addition, polynomial contrasts tested the linear and quadratic effect of PP inclusion levels.
Growth performance responses did not differ (P>0.05) between treatments. However, there was a trend (P=0.089) for improved FCR by 4.5% in PP-1000 compared to PP-0 at the finisher phase. In addition, trends for improved European production efficiency index were seen for PP-1000 during the finisher phase (P=0.087) and overall (P=0.057). Treatment PP-1000 had the highest carcass (P=0.030) and breast fillet yield (P=0.023). From the digestibility study, PP-1000 had higher AMEn (P=0.049) compared to treatments PP-2000 and PP-0.
The PP inclusion level resulted in higher breast (P=0.005), thigh (P=0.002) and liver (P=0.040) TAC. In particular, breast and thigh TAC increased in a quadratic pattern and reached plateu at PP-1000, whereas liver TAC continued to increase linearly. Gene expression of SOD was significantly up-regulated in the duodenum (P=0.027), jejunum (P=0.026) and ceca (P=0.023) in PP-1000 and PP-750 compared to PP-0. In ceca, CAT expression displayed a quadratic pattern of up-regulation (Pq=0.053) in the same direction with SOD (Pq=0.006).
Overall, this study provides evidence for potential PP-1000 benefits for carcass and breast fillet yield, energy sparing and overall antioxidant capacity in broiler gut and meat.
Researchers of this study
published in the proceedings of the scientific forum IPPE 2018
Konstantinos C. Mountzouris*, Vasileios Paraskeuas*, Eirini Griela*, Andreas Kern# and Konstantinos Fegeros*
*Department of Nutritional Physiology and Feeding, Agricultural University of Athens, 118 55 Athens, Greece
#Anco Animal Nutrition Competence GmbH, Linzer Strasse 55, 3100 Sankt Poelten, Austria